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Types of Hypothesis Testing and How to Perform Them in Python

Discover the different types of hypothesis tests and how to implement them in Python. This comprehensive guide helps beginners understand hypothesis testing concepts and perform them with Python code examples.
May 10, 2025
12 min read
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Types of Hypothesis Testing and How to Perform Them in Python

When you make a decision based on data, whether it's choosing which marketing strategy to pursue or predicting future trends in your business, you're engaging in a type of decision-making that is grounded in the scientific method. This is where hypothesis testing comes into play. At its core, hypothesis testing is the process by which we evaluate assumptions or claims about a population using sample data. In simpler terms, it’s about determining whether the data supports a particular belief or whether that belief should be rejected in favor of something new.

Why is this so crucial? Because every decision you make based on data comes with uncertainty. You might think that a new business strategy will boost your bottom line, or that a new drug is more effective than the old one, but how do you know for sure? Hypothesis testing provides the framework for making these types of decisions statistically — reducing the risk of being wrong.

Hypothesis testing isn’t just some theoretical exercise, either. It’s a tool widely used in various fields — from medicine (testing the effectiveness of new treatments) to economics (evaluating policy impacts) to business (understanding customer behavior). The flexibility of hypothesis testing lies in its universality — its ability to be applied to almost any data set, regardless of industry or problem.

But here’s the real kicker: you don't have to be a statistician to wield this power. Enter Python — the modern-day statistical powerhouse that turns complicated statistical tests into a few lines of code. Python libraries like SciPy and Statsmodels make it incredibly easy to conduct these tests and interpret results, all without having to manually crunch numbers. The power of statistical testing, once relegated to complex formulas and endless spreadsheets, is now in the hands of anyone willing to learn the basics of Python.

So, whether you're in business, healthcare, or social sciences, understanding hypothesis testing can help you make more informed, data-driven decisions. It’s about using the numbers, not just gut instinct, to guide your choices. And the best part? Python allows you to conduct these tests efficiently and effectively, putting statistical analysis in the hands of everyday decision-makers.

Let’s take a deeper dive into the different types of hypothesis tests — what they are, when to use them, and how Python can be your best friend in performing them with ease. From simple t-tests to the more complex ANOVA, we’re about to take the mystery out of hypothesis testing. Ready to get started? Let's go.

Parametric vs. Non-Parametric Tests: The Battle of Assumptions

When you’re diving into the world of hypothesis testing, the first thing you’ll notice is that not all statistical tests are created equal. Some tests assume certain conditions about your data, while others don’t. These assumptions determine whether a test is classified as parametric or non-parametric. Understanding the difference between these two types of tests is essential because it influences which test is appropriate to use in different situations.

Let’s break down what parametric and non-parametric tests are, and why they matter when conducting hypothesis tests.

Parametric Tests: The Data Whisperers

Parametric tests are the "gold standard" of hypothesis testing. Why? Because they rely on certain assumptions about the data, specifically that the data comes from a normal distribution and meets other criteria like homogeneity of variance (similar spread of data across groups). In other words, parametric tests assume that the underlying data fits a known distribution — typically a normal distribution (bell curve). The power of parametric tests lies in their precision and efficiency when the assumptions hold true.

When to Use Parametric Tests?

You’ll typically use a parametric test when:

  1. Your data is continuous and follows a normal distribution.
  2. The groups you are comparing have similar variances (this is especially relevant in two-sample tests).
  3. You have interval or ratio scale data, where numbers make sense in a real, ordered way (like height, weight, or income).

Examples of Parametric Tests

  • One-Sample t-test: Used to compare the mean of a single sample to a known value (e.g., comparing the average score of students to the class average).
  • Two-Sample t-test: Compares the means of two independent groups (e.g., comparing the test scores of two different teaching methods).
  • Paired t-test: Compares the means of two related groups (e.g., comparing pre-test and post-test scores for the same group of students).
  • Analysis of Variance (ANOVA): Compares means across three or more groups (e.g., comparing the effectiveness of three different diets on weight loss).

Advantages of Parametric Tests:

  • Efficiency: They are more powerful when the assumptions are met, meaning you are more likely to detect a true effect if it exists.
  • Precision: Because they make assumptions about the data, they tend to give more precise estimates of population parameters.

Disadvantages of Parametric Tests:

  • Assumption Dependency: The biggest drawback is that if the data doesn’t meet the assumptions (like normality), the results can be misleading. Violating the assumptions of normality or homogeneity of variance can lead to incorrect conclusions.

Non-Parametric Tests: The Freedom Seekers

On the flip side, non-parametric tests don’t require the data to follow a specific distribution (like the normal distribution) and are often referred to as distribution-free tests. These tests are more flexible and can be used with data that doesn’t meet the assumptions necessary for parametric tests. Non-parametric tests are particularly useful when you’re dealing with ordinal data (data that can be ranked but doesn’t have a precise numerical relationship) or when your data is not normally distributed.

In simpler terms, non-parametric tests are like the rebels of the statistical world: they don’t play by the rules of normality and can handle a wider range of data types.

When to Use Non-Parametric Tests?

You would consider using a non-parametric test when:

  1. Your data is not normally distributed (it may be skewed or have outliers).
  2. You have ordinal data (data that can be ranked but doesn’t have a meaningful distance between rankings).
  3. You’re working with nominal data (categories with no inherent order, like gender, color, or brand preference).

Examples of Non-Parametric Tests

  • Mann-Whitney U Test: This is a non-parametric alternative to the two-sample t-test. It compares the ranks of two independent groups instead of their means (e.g., comparing the effectiveness of two teaching methods when the data is skewed).
  • Wilcoxon Signed-Rank Test: A non-parametric alternative to the paired t-test, used for comparing the ranks of two related groups (e.g., comparing pre-test and post-test scores for the same group of students, but the data isn’t normally distributed).
  • Kruskal-Wallis Test: The non-parametric equivalent of one-way ANOVA, used to compare ranks across three or more groups (e.g., comparing customer satisfaction across three different product brands).
  • Chi-Squared Test: Used to test the relationship between two categorical variables (e.g., whether there is an association between gender and voting preference).

Advantages of Non-Parametric Tests:

  • Flexibility: They can be applied to data that doesn’t meet the assumptions required by parametric tests, including skewed or ordinal data.
  • Robustness: Non-parametric tests are generally more robust to outliers and non-normality in the data.
  • Easier to Use for Non-Continuous Data: Perfect for categorical, ranked, or ordinal data.

Disadvantages of Non-Parametric Tests:

  • Less Power: Non-parametric tests are usually less powerful than parametric tests if the data does meet the assumptions of parametric testing. They’re more likely to produce false negatives, meaning you might fail to detect a true effect when one exists.
  • Less Specific: Non-parametric tests tend to focus on the ranks or medians, rather than precise means, which can be less informative in some cases.

One-Sample Tests

A one-sample test is used when you want to determine if the mean of a sample differs significantly from a known or hypothesized population mean. This is useful when you have a sample and want to see if it matches a theoretical or known value for the entire population.

Example:
Imagine you are a teacher and you want to know if the average test score of your class (a sample) differs from the national average (population mean). A one-sample t-test can help you determine whether the average score of your class is significantly different from the known population average.

To perform a one-sample test using Python, you can use the scipy.stats.ttest_1samp function. Here's an example:

1. import numpy as np 
2. from scipy import stats 
3.   
4. # Sample data 
5. sample_data = [85, 90, 88, 91, 87, 92, 89, 94, 86, 906.   
7. # Population mean 
8. population_mean = 90 
9.  
10. # Perform one-sample t-test11. 
11. t_statistic, p_value = stats.ttest_1samp(sample_data, population_mean)
12.  
13. # Output results
14. print("T-statistic:", t_statistic)
15. print("P-value:", p_value)
16.  
17. if p_value < 0.05:
18. print("Reject the null hypothesis: The sample mean is significantly different from the population mean.")
19. else:
20. print("Fail to reject the null hypothesis: The sample mean is not significantly different from the population mean.")

Two-Sample Tests (Or Independent Samples Test)

The two-sample test is used when you want to compare the means of two independent groups to see if there’s a significant difference between them. This is often applied in experimental research where you compare two separate groups that are treated differently or exposed to different conditions.

Example:
Imagine you have two groups of students: one group is taught using traditional methods and the other with a new experimental method. You want to determine if there is a significant difference in the test scores between the two groups. You would use a two-sample t-test to evaluate if the means of the two groups are statistically different.

Use the scipy.stats.ttest_ind function to perform a two-sample test:

1. import numpy as np 
2. from scipy import stats 
3.   
4. # Sample data for two groups 
5. group_1 = [85, 90, 88, 91, 87, 92, 89, 94, 86, 906. group_2 = [78, 84, 80, 82, 76, 79, 81, 83, 77, 807.   
8. # Perform independent two-sample t-test 
9. t_statistic, p_value = stats.ttest_ind(group_1, group_2)
10.  
11. # Output results
12. print("T-statistic:", t_statistic)
13. print("P-value:", p_value)
14.  
15. if p_value < 0.05:
16. print("Reject the null hypothesis: There is a significant difference between the two groups.")
17. else:
18. print("Fail to reject the null hypothesis: There is no significant difference between the two groups.")

Paired Sample Test

Paired sample tests are used when you want to compare two related groups. This is often used in before-and-after studies, where you’re comparing the same group at two different times or conditions.

Example:
You want to know if a new treatment improves the test scores of students. You test the students before the treatment and then again after the treatment. Since the same students are involved, their pre- and post-treatment scores are "paired."

Use scipy.stats.ttest_rel for a paired sample test:

1. import numpy as np 
2. from scipy import stats 
3.   
4. # Sample data before and after treatment 
5. before_treatment = [78, 82, 85, 90, 88, 84, 77, 91, 89, 866. after_treatment = [85, 87, 88, 92, 91, 89, 86, 94, 93, 907.   
8. # Perform paired t-test 
9. t_statistic, p_value = stats.ttest_rel(before_treatment, after_treatment)
10.  
11. # Output results
12. print("T-statistic:", t_statistic)
13. print("P-value:", p_value)
14.  
15. if p_value < 0.05:
16. print("Reject the null hypothesis: The treatment significantly improved scores.")
17. else:
18. print("Fail to reject the null hypothesis: The treatment did not significantly improve scores.")

Here’s a helpful image that show the difference between One-Sample Tests, Independent Sample Test and Paired Sample Test

Difference between One-Sample Tests, Independent Sample Test and Paired Sample Test

Chi-Squared Test

Chi-squared tests are used for categorical data, particularly when you want to test for relationships between categorical variables. These tests can either assess the goodness of fit (whether a sample data matches an expected distribution) or independence (whether two categorical variables are independent).

Example:
You might want to test if there’s a relationship between gender and voting preference. In this case, you would use a Chi-squared test for independence.

Statistical Test:

  • Chi-squared test for independence: Tests whether two categorical variables are independent.
  • Chi-squared goodness of fit test: Tests whether the observed distribution of data fits an expected distribution.

Use scipy.stats.chisquare for goodness of fit or scipy.stats.chi2_contingency for independence testing:

1. import numpy as np 
2. from scipy import stats 
3.   
4. # Observed data (2x2 table for gender and voting preference) 
5. observed = np.array([[30, 10], [25, 15]]) 
6.   
7. # Perform chi-squared test for independence 
8. chi2_stat, p_value, dof, expected = stats.chi2_contingency(observed)
9.  
10. # Output results
11. print("Chi-squared statistic:", chi2_stat)
12. print("P-value:", p_value)
13.  
14. if p_value < 0.05:
15.  print("Reject the null hypothesis: There is a significant relationship between gender and voting preference.")
16. else:
17.  print("Fail to reject the null hypothesis: There is no significant relationship between gender and voting preference.")

This test checks whether gender and voting preference are independent, helping to understand the relationship between categorical variables.

ANOVA Test (Analysis of Variables)

ANOVA is used when you want to compare the means of three or more independent groups. This is helpful when you have more than two groups and want to know if there’s a significant difference between their means.

There’s many different types of ANOVA:

1. One-Way ANOVA:

  • When to use: You use One-Way ANOVA when you are comparing the means of three or more independent groups that are based on one independent variable (factor). This test helps determine if there is a statistically significant difference between the means of these groups.
  • Example: Comparing the average test scores of students from three different teaching methods (e.g., Traditional, Online, and Hybrid).
  • Null Hypothesis (H₀): All group means are equal.
  • Alternative Hypothesis (H₁): At least one group mean is different.

2. Two-Way ANOVA:

  • When to use: A Two-Way ANOVA is used when you are comparing the means of three or more groups based on two independent variables (factors). This test can help determine if there are any main effects of each factor and if there is an interaction effect between the two factors.
  • Example: Studying the effect of teaching method (Traditional vs. Online) and study environment (Quiet vs. Noisy) on students' test scores. In this case, you have two independent variables: teaching method and study environment, and you're interested in both their individual effects and their interaction.
  • Null Hypothesis (H₀):
    1. There is no effect of teaching method on test scores.
    2. There is no effect of study environment on test scores.
    3. There is no interaction between the teaching method and study environment.
  • Alternative Hypothesis (H₁): At least one of the above hypotheses is false.

Main Effects and Interaction Effect:

  • Main Effects: The direct effect of each factor on the dependent variable.
  • Interaction Effect: The combined effect of both factors together. In other words, it examines whether the effect of one factor depends on the level of the other factor.

3. Repeated Measures ANOVA:

  • When to use: Repeated Measures ANOVA is used when you have multiple measurements taken from the same subjects, at different time points or under different conditions. Essentially, it's used when the same group of subjects is tested multiple times.
  • Example: Testing how students' test scores change after three different teaching methods are implemented over three different time periods (e.g., pre-test, mid-test, and post-test).
  • Null Hypothesis (H₀): There is no difference in the means across the time points or conditions.
  • Alternative Hypothesis (H₁): There is a significant difference in the means across the time points or conditions.
  • Why use Repeated Measures ANOVA?: This test takes into account the correlation between measurements on the same subjects (since the same participants are measured multiple times), thus it provides more power compared to a standard one-way or two-way ANOVA where independent groups are used.

4. Multivariate Analysis of Variance (MANOVA):

  • When to use: MANOVA is an extension of ANOVA that allows for the analysis of multiple dependent variables simultaneously. It is used when you want to test the differences in means across three or more groups for multiple dependent variables.
  • Example: Testing the impact of different teaching methods on both test scores and student engagement (two dependent variables) across three teaching methods (Traditional, Online, and Hybrid).
  • Null Hypothesis (H₀): There is no difference in the means of the dependent variables across the groups.
  • Alternative Hypothesis (H₁): At least one of the dependent variables shows a significant difference across the groups.
  • Why use MANOVA?: MANOVA helps to assess the relationship between multiple dependent variables at once, reducing the risk of Type I errors that can occur when testing each dependent variable separately.

5. Mixed-Design ANOVA (or Split-Plot ANOVA):

  • When to use: A Mixed-Design ANOVA is used when you have both within-subjects and between-subjects factors. This means you have one factor where the same subjects are tested repeatedly (within-subjects), and another factor where different subjects are exposed to different levels of a treatment (between-subjects).
  • Example: Testing the effect of two types of diets (between-subjects) on weight loss over time (within-subjects). Participants are randomly assigned to one of the two diet groups, and their weight loss is measured at multiple time points (before, during, and after the diet).
  • Null Hypothesis (H₀): There are no significant effects for either the between-subjects factor (diet type) or the within-subjects factor (time) on weight loss.
  • Alternative Hypothesis (H₁): At least one factor or their interaction has a significant effect on weight loss.

Key Differences Between These Tests:

  • One-Way vs. Two-Way ANOVA:
    • One-Way ANOVA is used when you have one independent variable with three or more levels (groups), whereas Two-Way ANOVA is used when you have two independent variables.
  • Repeated Measures ANOVA vs. One-Way/Two-Way ANOVA:
    • Repeated Measures ANOVA is used when the same participants are measured multiple times (within-subjects), while One-Way and Two-Way ANOVA assume independent groups.
  • MANOVA vs. ANOVA:
    • MANOVA is used when you have multiple dependent variables, whereas ANOVA deals with a single dependent variable.

Here’s an example of One-Way test using scipy.stats.f_oneway

1. import numpy as np 
2. from scipy import stats 
3.   
4. # Sample data for three different teaching methods 
5. method_1 = [75, 80, 85, 90, 886. method_2 = [65, 70, 75, 80, 787. method_3 = [90, 92, 95, 96, 948.   
9. # Perform one-way ANOVA
10. f_statistic, p_value = stats.f_oneway(method_1, method_2, method_3)
11.  
12. # Output results
13. print("F-statistic:", f_statistic)14. print("P-value:", p_value)
15.  
16. if p_value < 0.05:
17. print("Reject the null hypothesis: There is a significant difference between the teaching methods.")
18. else:
19. print("Fail to reject the null hypothesis: There is no significant difference between the teaching methods.")

This ANOVA test compares the test scores of three different teaching methods to determine if the performance differences are statistically significant.


These are the most commonly used hypothesis tests, and Python’s rich ecosystem of libraries (like SciPy) makes performing these tests as easy as writing a few lines of code. Here’s a reference table of which Python libraries you can use for different hypothesis tests, including correlation tests:

A reference table of which Python libraries you can use for different hypothesis tests, including correlation tests

Conclusion: Making Informed Decisions with Hypothesis Testing

In conclusion, hypothesis testing is a critical tool in statistics that empowers us to make data-driven decisions with confidence. By understanding the different types of hypot hesis tests like null and alternative hypotheses, significance levels, p-values, and the different types of tests, you can apply statistical reasoning to real-world problems and uncover meaningful insights from data.

From comparing group means with t-tests to examining associations between categorical variables with Chi-squared tests, hypothesis testing provides a structured way to evaluate assumptions and make informed conclusions. Whether you’re testing a new marketing strategy, evaluating the effectiveness of a new product, or analyzing research data, hypothesis tests help you separate noise from true effects.

Moreover, as we saw with the various types of ANOVA, there’s a wealth of statistical tests tailored to different types of data and experimental designs. Whether you're comparing two groups, assessing repeated measures over time, or analyzing multiple dependent variables, there's a test for almost every situation.

As you continue to dive deeper into hypothesis testing, the importance of Python and its powerful libraries like SciPy and Statsmodels cannot be overstated. These tools make hypothesis testing accessible, turning complex statistical methods into easy-to-use functions with just a few lines of code. Python brings the power of statistics to anyone willing to learn and apply it, allowing data to inform decisions across industries and disciplines.

Remember, hypothesis testing isn't about proving something to be true; it's about assessing whether the data supports or contradicts your assumptions. By mastering these techniques, you're better equipped to make decisions based on evidence, improving the reliability and impact of your findings.

So, whether you're a business analyst, researcher, or student, continue exploring hypothesis testing, experiment with different tests, and leverage Python’s statistical tools to drive smarter decisions and discover new insights. The world of data is full of opportunities, and hypothesis testing is one of the most powerful ways to navigate it.

If you want to deepen your Python and statistical analysis skills, check out our Python for Data Science course on Skillcamper.

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